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■包装内容
His GraviTrap 层析柱（预装1 ml Ni-NTA Agarose）
10支
Ni-NTA Agarose 10ml
Guanidine HCl 50g
Urea 100g
1 M Imidazole, pH 8.0 50ml
5x Phosphate Buffer Stock Solution
(0.5 M NaH2PO4; pH 8.0) 100 ml
Control Vector DNA 1 μg
■产品特点
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Column material
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Polypropylene barrel, polyethylene frits |
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Medium |
Ni Sepharose 6 Fast Flow |
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Average bead size |
90 μm |
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Protein binding capacity
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Approx. 40 mg histidine-tagged protein/column |
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Bed volume |
1 ml |
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Compatibility during use |
Stable in all commonly used buffers, reducing agents, denaturants and detergents. (See Table 2.) |
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Avoid in buffers
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Chelating agents, e.g. EDTA, EGTA, citrate. |
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pH stability,short-term(2 h) |
2–14 |
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Storage |
20% ethanol |
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Storage temperature |
+4 to +30 ºC |
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■ 产品说明：
含10根装有1ml Ni Sepharose 6 FF凝胶的His Gravitrap预装柱和His Buffer kit；每注可处理10ml样品和吸附40mg His标记蛋白质；
蛋白纯化流程如下：
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